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A dinitro-functionalized metal-organic framework featuring visual and fluorogenic sensing of H2S in living cells, human blood plasma and environmental samples

Abstract

Here, we describe a new dinitro-functionalized Zr(IV) MOF (MOF = metal-organic framework) having UiO-66 (UiO = University of Oslo) framework topology called UiO-66-(NO2)2 (1). It shows fluorescence turn-on behavior towards H2S in simulated biological medium (HEPES buffer, pH = 7.4). By employing solvothermal conditions, 1 was successfully synthesized by reacting ZrCl4, H2BDC-(NO2)2 [H2BDC-(NO2)2 = 2,5-dinitro-1,4-benzenedicarboxylic acid] ligand and benzoic acid with a molar ratio of 1:1:10 in DMF (DMF = N,N-dimethylformamide) at 130 °C for 24 h. The material was characterized by infrared spectroscopy, X-ray powder diffraction (XRPD) and thermogravimetric (TG) analyses. The compound not only displays highly sensitive fluorometric sensing of H2S but also exhibits visually detectable colorimetric change towards H2S under day light. Moreover, the high selectivity of 1′ towards H2S is retained even when several other biologically intrusive species co-exist in the sensing medium. The limit of detection (LOD) of the compound is 12.39 µM which lies in the range of H2S concentration found in biological systems. Fluorescence microscopy studies in J774A.1 cells revealed the efficacy of the probe for imaging H2S in living cells. Moreover, this material can detect H2S in human blood plasma (HBP) and monitor sulfide concentration in real water samples. All these features clearly demonstrate that the material has huge potential for the highly selective sensing of both extracellular and intracellular H2S.

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Publication details

The article was received on 04 Dec 2017, accepted on 03 Feb 2018 and first published on 06 Feb 2018


Article type: Paper
DOI: 10.1039/C7AN01964E
Citation: Analyst, 2018, Accepted Manuscript
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    A dinitro-functionalized metal-organic framework featuring visual and fluorogenic sensing of H2S in living cells, human blood plasma and environmental samples

    S. Nandi, S. Banesh, V. Trivedi and S. Biswas, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C7AN01964E

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