Jump to main content
Jump to site search


An amplification-free electrochemical detection of exosomal miRNA-21 in serum samples

Abstract

Recent evidence suggests that small non-coding RNAs or microRNA (miRNA)s encapsulated in exosomes represent an important mechanism of communication between the cells. Exosomal miRNAs play an important role in oncogenesis via stimulating cell to cell communication and facilitating metastasis in cancers. Despite progressive advances, current methods for exosomal miRNA detection most rely on labor intensive sequencing approaches which are often prone to amplification bias and require costly and bulky equipment. Herein, we report an electrochemical approach for detection of cancer-derived exosomal miRNAs in human serum samples by selectively isolating the target miRNA using magnetic beads pre-functionalized with capture probes and then directly absorbing the targets onto the gold electrode surface. The level of adsorbed miRNA is detected electrochemically in the presence of the [Fe(CN)6]4-/3- redox system. This method enabled an excellent detection sensitivity of 1.0 pM with a relative standard deviation (%RSD) of <5.5% in cancer cells and serum samples (n = 8) collected from patients with colorectal adenocarcinoma. We believe that our approach could be useful for the quantification of exosomal miRNA in clinical samples.

Back to tab navigation

Supplementary files

Publication details

The article was received on 13 Nov 2017, accepted on 07 Feb 2018 and first published on 12 Feb 2018


Article type: Paper
DOI: 10.1039/C7AN01843F
Citation: Analyst, 2018, Accepted Manuscript
  •   Request permissions

    An amplification-free electrochemical detection of exosomal miRNA-21 in serum samples

    K. Boriachek, M. Umer, M. N. Islam, V. Gopalan, A. K. Lam, N. Nguyen and M. J. A. Shiddiky, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C7AN01843F

Search articles by author

Spotlight

Advertisements