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Compromise in uncertainty estimation by the modelling and validation approaches of an HPLC-UV method for vitamins A&E biochemical indicators measurement

Abstract

It is well admitted nowadays to associate the measurement uncertainty to the measurand, in order to judge the quality of a result related to the measurement process. However, the improvement of this parameter as well as the adaptation of its estimation modes, always remain an analytical challenge to be held, especially in chemical testing. In this paper, we outline a measurement uncertainty estimation mode based on the one-point linear calibration equation, to fully establishing the "Bottom-up" approach for estimating the measurement uncertainty of a multi-point calibration based HPLC-UV quantitative method. To demonstrate this estimation mode, we have followed as an example of interest, the influences resulting from the simultaneous determination of two biochemical indicators, namely the human plasma vitamers; retinol and α-tocopherol. Results from this estimation show consistent when compared to those obtained from the validation based alternative method, where the relative expanded uncertainties were found, at a 95% confidence level, less than (15%) for the low concentration ranges of the two molecules. However, the modeling approach brings all the desired interest of its use and shows quite achievable for comparative HPLC methods.

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Publication details

The article was received on 05 Nov 2017, accepted on 10 Feb 2018 and first published on 13 Feb 2018


Article type: Paper
DOI: 10.1039/C7AN01804E
Citation: Analyst, 2018, Accepted Manuscript
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    Compromise in uncertainty estimation by the modelling and validation approaches of an HPLC-UV method for vitamins A&E biochemical indicators measurement

    O. Djellouli, R. Eric, B. Dahmani and P. Hubert, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C7AN01804E

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