Jump to main content
Jump to site search


Rapid Resolution of Carbohydrate Isomers via Multi-site Derivatization Ion Mobility-Mass Spectrometry

Abstract

Identifying small sugar isomers can be challenging by ion mobility mass spectrometry (IM-MS) alone due to their small collision cross section differences. Herein, we report IM-MS results for multi-site, covalent carbohydrate derivatization with 3-carboxy-5-nitrophenylboronic acid (3C5NBA). Following reaction in aqueous solutions at room temperature , two molecules of 3C5NBA react with each mono- or disaccharide molecule to yield products that each have a distinguishable mobility signature. The reaction was rapid and resulted in detection of products immediately after 3C5NBA was mixed with the analyte. Eight disaccharides that varied in linkage, composition, and conformation ( or ) as well as four monosaccharides, D-glucose, D-galactose, D-mannose and D-fructose, were included in this study. The derivatives’ drift times showed significant shifts, with up to 3-fold gain in resolution when compared to previous literature reports. Moreover, the specific fragmentation (MS/MS) information gathered from these sugar derivatives provided further validation of the isomers’ structure for identification purposes.

Back to tab navigation

Supplementary files

Publication details

The article was received on 03 Nov 2017, accepted on 06 Jan 2018 and first published on 08 Jan 2018


Article type: Paper
DOI: 10.1039/C7AN01796K
Citation: Analyst, 2018, Accepted Manuscript
  •   Request permissions

    Rapid Resolution of Carbohydrate Isomers via Multi-site Derivatization Ion Mobility-Mass Spectrometry

    L. LI, K. R. McKenna, Z. LI, M. Yadav, R. Krishnamurthy, C. Liotta and F. Fernandez, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C7AN01796K

Search articles by author

Spotlight

Advertisements