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DNA microarray-based resonance light scattering assay for multiplexed detection of DNA mutation in papillary thyroid cancer

Abstract

Highly accurate analysis of single-nucleotide polymorphisms (SNPs) plays an important role in both of disease diagnostics and personalized medicine development. In this work, a DNA microarray-based resonance light scattering (RLS) assay has been developed for multiplexed detection of papillary thyroid carcinoma (PTC) related mutation points including BRAFV600E (t1m), NRAS codon 61 (t2m), TERT promoter g.1295228 (t31m) and TERT promoter g.1295250 (t32m) with high sensitivity and selectivity by attachment of polyvalent ssDNA modified 13 nm gold nanoparticles (ssDNAs@GNPs) followed by silver deposition for signal enhancement. The microarray-based RLS assay provides a detection limit (S/N = 3) at sub-nanomolar level for the target ssDNAs and determines allele frequencies as low as 0.2% for t1m, 0.2% for t2m, 0.5% for t31m, and 0.5% for t32m in the cocktail of target ssDNAs, respectively. The practicability of DNA microarray-based RLS assay is demonstrated by profiling of t2m in 50 clinical thyroid tissue samples of PTC patients, and satisfactory results are obtained.

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Supplementary files

Publication details

The article was received on 27 Oct 2017, accepted on 06 Jan 2018 and first published on 08 Jan 2018


Article type: Paper
DOI: 10.1039/C7AN01773A
Citation: Analyst, 2018, Accepted Manuscript
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    DNA microarray-based resonance light scattering assay for multiplexed detection of DNA mutation in papillary thyroid cancer

    Y. Wang, J. Gao, X. Meng and Z. Wang, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C7AN01773A

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