Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Tuesday 6th February 2018 from 11.00am to 11.15am (GMT).

During this time our website performance may be temporarily affected. If you have any questions please use the feedback button available under our menu button. We apologise for any inconvenience this might cause and thank you for your patience.



An upconversion nanoparticle-based fluorescence resonance energy transfer system for effectively sensing caspase-3 activity

Author affiliations

Abstract

We report a new fluorescence resonance energy transfer (FRET) sensing platform for the sensitive detection of caspase-3 activity in vitro and in cells using NaGdF4:Yb3+,Er3+@NaGdF4 upconversion nanoparticles (UCNPs) as the energy donor and Rhodamine B (RB) as the energy acceptor. The phosphorylated RB-modified peptide containing a caspase-3 cleavage site and cell-penetrating peptide (CPP) motif (sequence, (RB)-DEVDGGS(p)GCGT(p)GRKKRRQRRRPQ) is immobilized on the UCNP surface via the strong coordination interaction between Gd3+ ions with phosphate. After the cleavage of DEVD by caspase-3, the RB is released from the UCNP surface and the reduced upconversion luminescence (UCL) is recovered. Under the optimum conditions, the recovery ratio of the UCL is linearly dependent on the caspase-3 concentration within the range of 0.01 to 1000 pg mL−1 and with a limit of detection (LOD) of 0.01 pg mL−1 (S/N = 3). In particular, the as-proposed UCNP-based FRET sensing platform has reasonable selectivity which is successfully employed to monitor caspase-3 activity in drug-induced apoptosis of HeLa cells.

Graphical abstract: An upconversion nanoparticle-based fluorescence resonance energy transfer system for effectively sensing caspase-3 activity

Back to tab navigation

Supplementary files

Publication details

The article was received on 23 Oct 2017, accepted on 21 Dec 2017 and first published on 22 Dec 2017


Article type: Paper
DOI: 10.1039/C7AN01744H
Citation: Analyst, 2018, Advance Article
  •   Request permissions

    An upconversion nanoparticle-based fluorescence resonance energy transfer system for effectively sensing caspase-3 activity

    L. Liu, H. Zhang, D. Song and Z. Wang, Analyst, 2018, Advance Article , DOI: 10.1039/C7AN01744H

Search articles by author

Spotlight

Advertisements