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Collection of Amino Acids and DNA from Fingerprints using Hydrogels

Abstract

The amino acid profile obtained from a fingerprint can provide valuable information on its donor. For forensic scientists, recovering evidence relating to the amino acid profile of a suspect can potentially be valuable for identification and exclusion purposes. Herein we detail the use of cross linkable solutions of dextran-methacrylate to form hydrogels capable of collecting amino acids from surfaces followed by extraction and quantification with UPLC-MS. This method allows for non-marking amino acid profile analysis of fingerprints while allowing for their increased visualisation at a later stage using the standard method of cyanoacrylate fuming. We will demonstrate this method to also be capable of collecting DNA from fingerprints with a 20 - 60% yield in comparison to using a conventional cotton swab.

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Publication details

The article was received on 13 Oct 2017, accepted on 26 Dec 2017 and first published on 04 Jan 2018


Article type: Paper
DOI: 10.1039/C7AN01692A
Citation: Analyst, 2018, Accepted Manuscript
  • Open access: Creative Commons BY-NC license
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    Collection of Amino Acids and DNA from Fingerprints using Hydrogels

    W. van Helmond, V. O'Brien, R. de Jong, J. van Esch, S. Oldenhof and M. de Puit, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C7AN01692A

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