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Label-free detection of fibrinogen based on the fibrinogen-enhanced peroxidase activity of a fibrinogen–hemin composite

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Abstract

A simple, label-free colorimetric method for the determination of fibrinogen (Fib) in plasma is presented. In this work, it was observed that Fib interacted with hemin to form a hemin–Fib composite. Because Fib prevented hemin from the formation of m-oxo-dimers, the hemin–Fib composite possesses excellent peroxidase-like activity. Importantly, the peroxidase-like activity of Fib–hemin increased with the increase in the Fib. This allows us to utilize the H2O2–ABTS colorimetric system for the quantitative analysis of Fib. This optimized method provided a linear determination range of 2.0–100 pM with a correlation of 0.9975. The limit of detection for Fib was experimentally determined to be 0.7 pM based on a signal-to-noise ratio (S/N) of 3. This novel approach provides a rapid, sensitive, cost efficient and robust bioassay for detection of Fib in pathology and clinical applications.

Graphical abstract: Label-free detection of fibrinogen based on the fibrinogen-enhanced peroxidase activity of a fibrinogen–hemin composite

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Publication details

The article was received on 08 Oct 2017, accepted on 16 Dec 2017 and first published on 19 Dec 2017


Article type: Paper
DOI: 10.1039/C7AN01661A
Citation: Analyst, 2018, Advance Article
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    Label-free detection of fibrinogen based on the fibrinogen-enhanced peroxidase activity of a fibrinogen–hemin composite

    T. Hou, Y. Zhang, T. Wu, M. Wang, Y. Zhang, R. Li, L. Wang, Q. Xue and S. Wang, Analyst, 2018, Advance Article , DOI: 10.1039/C7AN01661A

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