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Laser-induced Surface Deformation Microscope for the Study of the Dynamic Viscoelasticity of Plasma Membrane in a Living Cell

Abstract

The laser-induced surface deformation (LISD) microscope is developed and is applied to the measurements on the dynamic relaxation responses of plasma membrane in a living cell. A laser beam is tightly focused onto an optional area of cell surfaces and the focused light induces microscopic deformation on the surface with radiation pressure. The LISD microscope not only allows non-contact and destruction-free measurements but provides the power spectra of the surface responses depending on the frequency of the intensity of the laser beam. An optical system for the LISD is equipped to a microscope, allowing us to measure the relaxation responses in the sub-cellular-sized regions of plasma membrane. In addition, the forced oscillation by the radiation pressure for the surface deformation extends the upper limit of the frequency range in the obtained power spectra to 106 Hz, which enables us to measure the relaxation responses in the local regions within plasma membrane. From the differences in power-law exponents at higher frequencies, it is clarified that a cancerous cell obeys a weaker single power-law than a normal fibroblast cell. Furthermore, the power spectrum of a keratinocyte cell obeys the power-law with two exponents, indicating that alternative mechanical models to a conventional soft glassy rheology model, where single power-laws explain cells’ responses below about 103 Hz, are needed for their understanding in wider frequency range. The LISD microscope would contribute to investigate microscopic cell rheology, which is important for clarifying mechanisms of cell migration and tissue construction.

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Publication details

The article was received on 29 Sep 2017, accepted on 11 Apr 2018 and first published on 13 Apr 2018


Article type: Paper
DOI: 10.1039/C7AN01620D
Citation: Analyst, 2018, Accepted Manuscript
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    Laser-induced Surface Deformation Microscope for the Study of the Dynamic Viscoelasticity of Plasma Membrane in a Living Cell

    T. Morisaku and H. Yui, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C7AN01620D

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