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Affinity resins as new tools for identifying target proteins of ascorbic acid

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Abstract

L-Ascorbic acid (AA) has diverse physiological functions, but little is known about the functional mechanisms of AA. In this study, we synthesized two types of affinity resin on which AA is immobilized in a stable form to identify new AA-targeted proteins, which can provide important clues for elucidating unknown functional mechanisms of AA. To our knowledge, an affinity resin on which AA as a ligand is immobilized has not been prepared, because AA is very unstable and rapidly degraded in an aqueous solution. By using the affinity resins, cytochrome c (cyt c) was identified as an AA-targeted protein, and we showed that oxidized cyt c exhibits specific affinity for AA. These results suggest that two kinds of AA-affinity resin can be powerful tools to identify new target proteins of AA.

Graphical abstract: Affinity resins as new tools for identifying target proteins of ascorbic acid

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Publication details

The article was received on 25 Sep 2017, accepted on 26 Dec 2017 and first published on 09 Jan 2018


Article type: Paper
DOI: 10.1039/C7AN01592E
Citation: Analyst, 2018, Advance Article
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    Affinity resins as new tools for identifying target proteins of ascorbic acid

    Y. Iwaoka, K. Nishino, T. Ishikawa, H. Ito, Y. Sawa and A. Tai, Analyst, 2018, Advance Article , DOI: 10.1039/C7AN01592E

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