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Issue 3, 2018
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Competitive annealing mediated isothermal amplification of nucleic acids

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Abstract

A novel nucleic acid isothermal amplification method with high specificity, efficiency and rapidity was developed. The principle of primer design was achieved by slight modification of PCR primers. Acceleration probes were also explored. The newly developed method is of great potential for point of care diagnostics.

Graphical abstract: Competitive annealing mediated isothermal amplification of nucleic acids

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Publication details

The article was received on 21 Sep 2017, accepted on 16 Dec 2017 and first published on 19 Dec 2017


Article type: Communication
DOI: 10.1039/C7AN01569K
Citation: Analyst, 2018,143, 639-642
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    Competitive annealing mediated isothermal amplification of nucleic acids

    R. Mao, L. Qi, J. Li, M. Sun, Z. Wang and Y. Du, Analyst, 2018, 143, 639
    DOI: 10.1039/C7AN01569K

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