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Direct detection of lysine side chain NH3+ in protein–heparin complexes using NMR spectroscopy

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Abstract

Two NMR observables, the NζH3+ peak in the HISQC spectrum and Nζ chemical shift difference between the free and heparin-bound forms, can identify binding-interface lysines in protein–heparin complexes. Unlike backbone chemical shifts, these direct probes are stringent and are less prone to either false positives or false negatives.

Graphical abstract: Direct detection of lysine side chain NH3+ in protein–heparin complexes using NMR spectroscopy

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Publication details

The article was received on 24 Aug 2017, accepted on 13 Dec 2017 and first published on 13 Dec 2017


Article type: Communication
DOI: 10.1039/C7AN01406F
Citation: Analyst, 2018, Advance Article
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    Direct detection of lysine side chain NH3+ in protein–heparin complexes using NMR spectroscopy

    K. M. Sepuru, J. Iwahara and K. Rajarathnam, Analyst, 2018, Advance Article , DOI: 10.1039/C7AN01406F

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