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Issue 2, 2018
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Real-time CRP detection from whole blood using micropost-embedded microfluidic chip incorporated with label-free biosensor

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Abstract

We demonstrate the detection of C-creative protein (CRP) from whole blood samples without sample pretreatment by using a lab-on-a-chip system consisting of a microfluidic chip and a label-free biosensor. The microfluidic chip includes an array of microposts for filtering blood cells and allows only plasma to flow through and reach the guided-mode resonance (GMR) biosensor for real-time monitoring. The developed GMR sensor can achieve a bulk sensitivity of 186 nm RIU−1, which supports a limit of detection of 3.2 ng mL−1 for recombinant CRP spiked in human serum. The results are comparable with those obtained using enzyme-linked immunosorbent assay. In addition, we demonstrate the efficacy of filtration of blood cells using microposts and simultaneous measurement of CRP concentration using a GMR sensor by using whole blood and plasma samples.

Graphical abstract: Real-time CRP detection from whole blood using micropost-embedded microfluidic chip incorporated with label-free biosensor

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Publication details

The article was received on 18 Aug 2017, accepted on 16 Dec 2017 and first published on 19 Dec 2017


Article type: Paper
DOI: 10.1039/C7AN01374D
Citation: Analyst, 2018,143, 503-510
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    Real-time CRP detection from whole blood using micropost-embedded microfluidic chip incorporated with label-free biosensor

    M. Tsai, C. Hsiung, Y. Chen, C. Huang, H. Hsu and P. Hsieh, Analyst, 2018, 143, 503
    DOI: 10.1039/C7AN01374D

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