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Quantitative Raman microspectroscopy for water permeability parameters at a droplet interface bilayer

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Abstract

Using confocal Raman microspectroscopy, we derive parameters for bilayer water transport across an isolated nanoliter aqueous droplet pair. For a bilayer formed with two osmotically imbalanced and adherent nanoliter aqueous droplets in a surrounding oil solvent, a droplet interface bilayer (DIB), the water permeability coefficient across the lipid bilayer was determined from monitoring the Raman scattering from the C[triple bond, length as m-dash]N stretching mode of K3Fe(CN)6 as a measure of water uptake into the swelling droplet of a DIB pair. We also derive passive diffusional permeability coefficient for D2O transport across a droplet bilayer using O–D Raman signal. This method provides a significant methodological advance in determining water permeability coefficients in a convenient and reliable way.

Graphical abstract: Quantitative Raman microspectroscopy for water permeability parameters at a droplet interface bilayer

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Publication details

The article was received on 13 Aug 2017, accepted on 22 Dec 2017 and first published on 04 Jan 2018


Article type: Paper
DOI: 10.1039/C7AN01349C
Citation: Analyst, 2018, Advance Article
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    Quantitative Raman microspectroscopy for water permeability parameters at a droplet interface bilayer

    S. Braziel, K. Sullivan and S. Lee, Analyst, 2018, Advance Article , DOI: 10.1039/C7AN01349C

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