Issue 3, 2018

Quantitative Raman microspectroscopy for water permeability parameters at a droplet interface bilayer

Abstract

Using confocal Raman microspectroscopy, we derive parameters for bilayer water transport across an isolated nanoliter aqueous droplet pair. For a bilayer formed with two osmotically imbalanced and adherent nanoliter aqueous droplets in a surrounding oil solvent, a droplet interface bilayer (DIB), the water permeability coefficient across the lipid bilayer was determined from monitoring the Raman scattering from the C[triple bond, length as m-dash]N stretching mode of K3Fe(CN)6 as a measure of water uptake into the swelling droplet of a DIB pair. We also derive passive diffusional permeability coefficient for D2O transport across a droplet bilayer using O–D Raman signal. This method provides a significant methodological advance in determining water permeability coefficients in a convenient and reliable way.

Graphical abstract: Quantitative Raman microspectroscopy for water permeability parameters at a droplet interface bilayer

Article information

Article type
Paper
Submitted
13 Aug 2017
Accepted
22 Dec 2017
First published
04 Jan 2018

Analyst, 2018,143, 747-755

Quantitative Raman microspectroscopy for water permeability parameters at a droplet interface bilayer

S. Braziel, K. Sullivan and S. Lee, Analyst, 2018, 143, 747 DOI: 10.1039/C7AN01349C

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