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Magnetic bead-based peptide extraction methodology for tissue imaging

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Abstract

MALDI-TOF imaging mass spectrometry (IMS) is a common technique used for analyzing tissue samples, as it allows the user to detect multiple different analytes simultaneously. However, the detection and analysis of these analytes may sometimes be hampered due to the presence of contaminants in the tissue microenvironment, which leads to ion suppression. This challenge necessitates the development of an active extraction technique to selectively isolate analytes of interest without compromising their spatial localization within a tissue sample. This study proposes a magnetic bead-based active extraction approach to selectively sequester peptides of interest from tissue samples. The technique utilizes a heterobifunctional cross-linker to covalently bind peptides with free primary amine groups to functionalized magnetic beads. The cross-linked peptides can then be collected using a transfer magnet and imaged using MALDI-TOF IMS. We have established that this technique not only successfully isolates peptides both in-solution and on a solid surface, but also extracts peptides from a tissue section without significantly compromising their spatial localization. This novel method provides the means to detect a unique subset of peptides from tissue sections when compared to unextracted tryptically digested tissue, all while minimizing the presence of contaminants and maintaining spatial localization.

Graphical abstract: Magnetic bead-based peptide extraction methodology for tissue imaging

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Publication details

The article was received on 07 May 2017, accepted on 31 Oct 2017 and first published on 03 Nov 2017


Article type: Paper
DOI: 10.1039/C7AN00757D
Citation: Analyst, 2018, Advance Article
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    Magnetic bead-based peptide extraction methodology for tissue imaging

    W. T. Andrews, S. B. Skube and A. B. Hummon, Analyst, 2018, Advance Article , DOI: 10.1039/C7AN00757D

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