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Quantitative Proteomic Analysis of HeLa Cells in Response to Biocompatible Fe2C@C Nanoparticles: 16O/18O-Labeled & HPLC-ESI-Orbit-Trap Profiling Approach

Abstract

The effective detection of molecular biomarker such as proteins, lipids, carbohydrates, and pathogens in living body is a huge challenge in the field of nanomedicine. Here, we have investigated the comparative quantitative proteomics analysis of molecular response of HeLa cells to biocompatible Fe2C@C nanoparticles (NPs) using 16O/18O isotopes labelling in cell culture. The relative binding efficiency of proteins to Fe2C@C NPs was calculated. The HPLC-ESI-orbit-trap analysis found 51 differentially expressed proteins out of which 23 were over expressed and 28 down regulated. This study showed that Fe2C@C NPs altered the expression of proteins involved in endocytosis, cell-cycle regulation and cell membrane protrusions. Further, quantification and validation of mass spectrometry (MS) results was successfully confirmed by Western blot analysis of cytochrome C. The change in expression of proteins can be useful for early stage disease diagnoses and the development of tailored therapeutic strategies. This study is the first large-scale characterization of low abundance proteins on Fe2C@C NPs, providing the biochemical basis for the assessment of suitability of magnetic NPs as biomedical marker and emerging functional probes.

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Publication details

The article was received on 18 Sep 2017, accepted on 07 Nov 2017 and first published on 08 Nov 2017


Article type: Paper
DOI: 10.1039/C7TX00248C
Citation: Toxicol. Res., 2017, Accepted Manuscript
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    Quantitative Proteomic Analysis of HeLa Cells in Response to Biocompatible Fe2C@C Nanoparticles: 16O/18O-Labeled & HPLC-ESI-Orbit-Trap Profiling Approach

    M. Hasan, G. Mustafa, J. Iqbal, M. Ashfaq and N. Mahmood, Toxicol. Res., 2017, Accepted Manuscript , DOI: 10.1039/C7TX00248C

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