Jump to main content
Jump to site search


Neurochemical characterization of nerve fibers in the porcine gallbladder wall under physiological conditions and after administration of Salmonella Enteritidis lipopolysaccharides (LPS)

Abstract

Lipopolysaccharide (LPS, bacterial endotoxin) is a component of the cellular membrane of Gram-negative bacteria, which is known as an important pathological factor. In spite of many previous studies describing multidirectional negative effects of LPS on the living organism, knowledge concerning the influence of bacterial endotoxin on the gallbladder innervation is extremely scarce. The present study, based on immunofluorescence technique, describes changes in neurochemical characterization of nerves within various parts of the porcine gallbladder (neck, body and fundus) after the administration of low doses of LPS. The obtained results show that even low doses of bacterial endotoxin affect the nerve structures within the gallbladder wall and the intensity of fluctuations in immunoreactivity to particular substances clearly depends on the part of the investigated organ. The most evident changes were observed in the case of fibers exhibiting the presence of neuropeptide Y (the increase from 7.84±0.17 to 14.66±0.37) in neck, substance P (the increase from 0.88±0.1 to 8.4±0.3) in body and vesicular acetylocholine transporter in gallbladders fundus (the increase from 4.29±0.18 to 11.01±0.26). The mechanisms of observed changes still remain unclear, but probably they are connected with the pro-inflammatory and/or neurodegenerative activity of LPS.

Back to tab navigation

Publication details

The article was received on 28 Jul 2017, accepted on 24 Oct 2017 and first published on 26 Oct 2017


Article type: Paper
DOI: 10.1039/C7TX00211D
Citation: Toxicol. Res., 2017, Accepted Manuscript
  •   Request permissions

    Neurochemical characterization of nerve fibers in the porcine gallbladder wall under physiological conditions and after administration of Salmonella Enteritidis lipopolysaccharides (LPS)

    K. Makowska, A. Mikolajczyk, J. Calka and S. Gonkowski, Toxicol. Res., 2017, Accepted Manuscript , DOI: 10.1039/C7TX00211D

Search articles by author

Spotlight

Advertisements