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Protective Effects of Nigella sativa lin Seed Extract on Lead Induced Neurotoxicity during Development and Early Life in Mice Model

Abstract

Lead (Pb), a ubiquitous heavy metal and a known neurotoxicant, produces adverse effects on brain via increased production of reactive oxygen species (ROS) and causes oxidative stress. In this study we examined the neuroprotective effects of ethanolic extract of Nigella sativa lin seeds on Pb induced oxidative stress in developing brain of mice. Mice pups were exposed to low (0.1%) and high (0.2%) dose of Pb from first day of pregnancy through their mothers (via drinking water) and lactation till post-natal day (PND) 21. mRNA expression level of superoxide dismutase (SOD1) peroxiredoxin (Prdx6), amyloid precursor protein (APP) common, APP 695 and APP 770 were examined in cortex and hippocampus of mice brain excised on PND 21 by semi quantitative RT-PCR. The free radical scavenging activity of ethanolic Nigella sativa lin extract was assessed by DPPH assay. Results showed that Pb exposure caused significant decrease in expression of SOD1, Prdx6, APP 695 and increase in APP 770 in both cortex and hippocampus in a dose dependent manner as compared to control group. The expression of APP common remained unaltered. Histological assessment of cortex and hippocampus demonstrated a decrease neuronal number and Nissl bodies. The administration of 250 and 500 mg/kg ethanolic Nigella sativa lin extract reversed the adverse effects by significantly increasing the expression of SOD1, Prdx6 and APP695 and decreasing the expression of APP770 in both the regions. These results strongly suggest that Nigella sativa lin supplementation greatly improves Pb-induced neurotoxicity in early life and possess neuroprotective and antioxidant potential.

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Publication details

The article was received on 20 Jul 2017, accepted on 04 Oct 2017 and first published on 05 Oct 2017


Article type: Paper
DOI: 10.1039/C7TX00201G
Citation: Toxicol. Res., 2017, Accepted Manuscript
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    Protective Effects of Nigella sativa lin Seed Extract on Lead Induced Neurotoxicity during Development and Early Life in Mice Model

    U. J. Butt, S. A. A. Shah, T. Ahmed and S. Zahid, Toxicol. Res., 2017, Accepted Manuscript , DOI: 10.1039/C7TX00201G

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