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Effect of bisphenol A on testicular steroidogenesis and its amelioration by quercetin: in vivo and in silico approach

Abstract

Bisphenol A (BPA), a phenyl ring containing synthetic xenoestrogen, is widely used in manufacture of polycarbonate plastics, epoxy resins and as a non-polymer additive to other plastics. Food is considered as the main source of exposure to BPA as it leaches out from the food containers as well as surface coatings. It causes toxicity in liver, kidney, brain, and other organs by initiating the process of lipid peroxidation. The present investigation was an attempt to evaluate effect of BPA on steroidogenesis and its amelioration by quercetin. Inbred Swiss strain male albino mice were orally administered with 80, 120 and 240 mg/kg body weight/day of BPA for 45 Days. The results revealed that BPA cause significant (p<0.05) and dose-dependent changes in body weight and biochemical parameters like protein, cholesterol, lipid contents as well as activies of 3β-and 17β-hydroxysteroid dehydrogenases in testis of mice. It was also found to significantly reduce testosterone level in serum. Oral administration of quercetin (30, 60 and 90 mg/kg body weight/day) along with high dose of BPA (240 mg/kg body weight/day) for 45 days caused significant amelioration in body weight and steroidogenesis as compared to BPA alone treated group. The effect was dose-dependent. This amelioration in BPA-induced toxicity might be due to its antioxidative property of quercetin. The reduction in the function of enzymes was confirmed by in silico bindings. BPA and quercetin shows competitive binding with steroidogenic enzymes as well as they binds with each other.This could be possible mechanism to reduce the toxic effect of BPA which have been supported by molecular dynamics simulations for the molecular level recognition with structural insights.

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Publication details

The article was received on 03 Jun 2017, accepted on 13 Sep 2017 and first published on 14 Sep 2017


Article type: Paper
DOI: 10.1039/C7TX00161D
Citation: Toxicol. Res., 2017, Accepted Manuscript
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    Effect of bisphenol A on testicular steroidogenesis and its amelioration by quercetin: in vivo and in silico approach

    R. J. Verma, S. Samova, C. Patel, H. Doctor and H. A. Pandya, Toxicol. Res., 2017, Accepted Manuscript , DOI: 10.1039/C7TX00161D

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