Jump to main content
Jump to site search


Effects of ambient PM2.5 and 9-nitroanthracene on DNA damage and repair, oxidative stress and metabolic enzymes in lungs of rats

Abstract

Ambient fine particulate matter (PM2.5) is a complex mixture associated with lung cancer risk. PM2.5-bound nitro-polycyclic aromatic hydrocarbons (NPAHs) have been demonstrated that they possess mutagenicityand carcinogenicity. Previous studies showed that PM2.5 induced DNA damage, whereas there is little knowledge of whether 9-nitroanthracene (9-NA), a typical compound of NPAHs in PM2.5, cause DNA damage. Also, the regulating mechanisms of PM2.5 and 9-NA on DNA damage and repair are not yet fully established. Here we sought to investigate the molecular mechanisms of DNA damage and repair in lungs of male Wistar rats exposed to PM2.5 (1.5 mg/kg body weight) or three different dosages of 9-NA. And then DNA strand breaks, 8-OH-dG formation, DNA-protein crosslink and DNA repair gene expressions in rat lungs were analyzed. In addition, alteration in oxidative stress factors and metabolic enzymes were detected. The results showed that (1) PM2.5 and higher dosage 9-NA (4.0 × 10-5 and 1.2 × 10-4 mg/kg body weight) significantly caused lung DNA damage, accompanied by increasing OGG1 expression while inhibiting MTH1 and XRCC1 expression, elevating the levels of GADD153, hemeoxygenase-1 and malondialdehyde, and promoting the activities of CYP450 isozymes and glutathione S-transferase. (2) 1.3 × 10-5 mg/kg 9-NA exposure couldn’t cause DNA damage and oxidative stress. (3) Under the approximately equivalent dose level, PM2.5-induced DNA damage effects were more obvious than 9-NA with positive correlation. It suggests that DNA damage caused by PM2.5 and 9-NA may be mediated partially through influencing DNA repair capacity and enhancing oxidative stress and biotransformation, and this negative effect of 9-NA might be related to PM2.5-induced lung genotoxicity.

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 01 Mar 2017, accepted on 16 May 2017 and first published on 18 May 2017


Article type: Paper
DOI: 10.1039/C7TX00065K
Citation: Toxicol. Res., 2017, Accepted Manuscript
  •   Request permissions

    Effects of ambient PM2.5 and 9-nitroanthracene on DNA damage and repair, oxidative stress and metabolic enzymes in lungs of rats

    R. Li, L. Zhao, L. Zhang, M. Chen, J. Shi, C. Dong and Z. Cai, Toxicol. Res., 2017, Accepted Manuscript , DOI: 10.1039/C7TX00065K

Search articles by author