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Real-time fluorescence turn-on assay for acetylcholinesterase activity based on the controlled release of a perylene probe from the MnO2 nanosheets

Abstract

We exploit a real-time perylene probe fluorescence turn-on method to detect acetylcholinesterase (AChE) activity through the selective decomposition of the MnO2 nanosheets. The surface of the MnO2 nanosheets was negatively charged. The perylene probe (P-4C+) had four positively charged quaternary ammonium groups. When mixed them together, P-4C+ was attached to the surface of the MnO2 nanosheets through electrostatic attractive interactions. The fluorescence of P-4C+ was effectively quenched by the MnO2 nanosheets. Acetylthiocholine (ATCh) could be hydrolyzed to thiocholine by AChE. Thiocholine is a reducing agent. It could reduce MnO2 nanosheets to Mn2+ and thus triggered the decomposition of the MnO2 nanosheets. As a result, P-4C+ was released and the fluorescence of P-4C+ was restored. AChE inhibitor would restrain the catalytic activity of AChE and resulted in a reduced fluorescence recovery of P-4C+. Our assay method is simple, selective, with low toxicity and better biocompatibility, which would facilitate the AChE activity associated biological and biomedical applications.

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Publication details

The article was received on 12 Nov 2016, accepted on 09 Apr 2017 and first published on 10 Apr 2017


Article type: Paper
DOI: 10.1039/C6TC04938A
Citation: J. Mater. Chem. C, 2017, Accepted Manuscript
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    Real-time fluorescence turn-on assay for acetylcholinesterase activity based on the controlled release of a perylene probe from the MnO2 nanosheets

    Y. Zhang, C. Zhang, J. Chen, Y. Li, M. Yang, H. Zhou, S. A. Shahzad, H. Qi, C. Yu and S. Jiang, J. Mater. Chem. C, 2017, Accepted Manuscript , DOI: 10.1039/C6TC04938A

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