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Novel peptides for small-caliber graft functionalization selected by a phage display of endothelial-positive/platelet-negative combined selection

Abstract

Coronary artery disease is the leading cause of death in the world. While autologous vessels transplantation remains a viable option, often patients may have a limited availability of suitable veins/arteries. Nowadays, although the efforts of research towards developing synthetic vessels, the small-diameter conduits have not fully met clinical needs. Surface modifications to mimic the vascular wall or seeding of endothelial cells within the lumen of the graft prior implantation are common approaches. However, due to difficulties of the seeding methods, the immobilization of short recognition sequences presenting cell binding motifs is remain one of the most promising strategies. In this paper, about 600 endothelial cell specific peptides have been reviewed and compared with three sequences selected by an improved phage display biopanning protocol. Indeed, the standard protocol has been ameliorated by (i) a positive selection of Endothelial Progenitor Cells (EPCs)-binding phage clones, (ii) a negative selection against platelets binding phage clones, and (iii) the introduction of phage binding index (PBI), which is a target affinity scoring function. The selected peptides were used to modify ePTFE surface, and our results indicated that the peptide modified surfaces may be useful to solve the problems of small calibre grafts.

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Publication details

The article was received on 05 Oct 2017, accepted on 08 Nov 2017 and first published on 09 Nov 2017


Article type: Paper
DOI: 10.1039/C7TB02652H
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    Novel peptides for small-caliber graft functionalization selected by a phage display of endothelial-positive/platelet-negative combined selection

    M. C. Munisso and T. Yamaoka, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB02652H

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