Jump to main content
Jump to site search


Cervical Cancer Long-term G1 Cell Cycle Arrest Induced by Co-Immobilized TNF-α Plus IFN-γ Polymeric Drugs

Abstract

A realistic control of cell cycle arrest is an attractive goal for the development of new effective anti-cancer drugs. Any clinical application of an effective anti-cancer drug necessarily relies on an understanding of interacting cellular mechanisms. In the present study, we prepared a co-immobilized TNF-α plus IFN-γ biomaterial, which showed to have a significant inhibition effect on cervical cancer cell growth, as demonstrated by a series of structural and cellular characterizations. We found that co-immobilized TNF-α plus IFN-α induced a long-term arrest of the cell cycle in the G1 phase in HeLa, SiHa, and CaSki cells, respectively. More surprisingly, the expression level of the p27 protein decreased, even when p27 mRNA was highly expressed. In addition, the gene-chip results and microarray analysis showed that p57 may be downstream from p27, that acts as a direct regulator of the long-term cell cycle arrest at G1 in these cells, leaving no escape for cervical cancer cells. Finally, we also investigated the anti-tumor mechanism of co-immobilized TNF-α plus IFN-γ in vivo, using nude mice animal model. To sum up, our findings suggested that the co-immobilized TNF-α plus IFN-γ can induce a long-term cell cycle arrest in cancer, thus serve as a very efficient tool for treating cervical cancer.

Back to tab navigation

Publication details

The article was received on 29 Sep 2017, accepted on 05 Dec 2017 and first published on 05 Dec 2017


Article type: Paper
DOI: 10.1039/C7TB02608K
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
  •   Request permissions

    Cervical Cancer Long-term G1 Cell Cycle Arrest Induced by Co-Immobilized TNF-α Plus IFN-γ Polymeric Drugs

    W. Chen, W. Wang, L. Chen, J. Chen, X. Lu, Z. Li, B. Wu, L. Yin and Y. Guan, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB02608K

Search articles by author

Spotlight

Advertisements