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Issue 45, 2017
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SU-8 based pyrolytic carbon for the electrochemical detection of dopamine

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Abstract

Here we investigated the electrochemical properties and dopamine (DA) detection capability of SU-8 photoresist based pyrolytic carbon (PyC) as well as its biocompatibility with neural cells. This approach is compatible with microfabrication techniques which is crucial for device development. X-ray photoelectron spectroscopy shows that PyC consists 98.5% of carbon, while oxygen plasma treatment (PyC-O2) increases the amount of oxygen up to 27.1%. PyC showed nearly reversible (ΔEp 63 mV) electron transfer kinetics towards outer sphere redox probe (Ru(NH3)62+/3+), while the reaction on PyC-O2 was quasi-reversible (ΔEp > 75 mV). DA showed both diffusion and adsorption-defined reaction kinetics with fast electron transfer with the ΔEp values of 50 mV and 30 mV, for PyC and PyC-O2, respectively. The strong interaction between the hydroxyl groups on the surface and DA, as confirmed by simulations, facilitates the redox reactions of DA. DA showed a linear response in the measured physiologically relevant range (50 nM–1 μM) and sensitivities were 1.2 A M−1 cm−2 for PyC and 2.7 A M−1 cm−2 for PyC-O2. Plasma oxidation (PyC-O2) improved cell adhesion even more than poly-L-lysine (PLL) coating on PyC, but best adhesion was achieved on PLL coated PyC-O2. Glial cells, neuroblastoma cells and neural stem cells all showed similar behavior.

Graphical abstract: SU-8 based pyrolytic carbon for the electrochemical detection of dopamine

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Publication details

The article was received on 14 Sep 2017, accepted on 01 Nov 2017 and first published on 01 Nov 2017


Article type: Paper
DOI: 10.1039/C7TB02469J
Citation: J. Mater. Chem. B, 2017,5, 9033-9044
  • Open access: Creative Commons BY-NC license
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    SU-8 based pyrolytic carbon for the electrochemical detection of dopamine

    E. Peltola, J. J. Heikkinen, K. Sovanto, S. Sainio, A. Aarva, S. Franssila, V. Jokinen and T. Laurila, J. Mater. Chem. B, 2017, 5, 9033
    DOI: 10.1039/C7TB02469J

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