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Collagen structure regulates MSCs behavior by MMPs involved cell-matrix interactions


Various scaffolds are studied to form cell niches and regulate mesenchymal stem cells (MSCs) behaviors. Collagen serves as one of the most promising materials for tissue engineering, but the cell-matrix interactions between MSCs and collagen are still poorly understood. In this work, we prepared methacrylated collagen (CMA) and gelatin (GMA) to form photo cross-linking hydrogels. The structure, morphology, mechanical properties and degradation behaviors of the derivatives and hydrogels were characterized, finding that the advanced structure was the major difference between collagen and gelatin hydrogels. MSCs were encapsulated in the hydrogels and cultured for 14 days in vitro, with or without the tissue inhibitor of metalloproteinase (TIMP). The CCK-8 and CLSM demonstrated that the cells in CMA hydrogels showed better spreading and proliferation than those in GMA hydrogels. The qRT-PCR and quantitative protein assay verified the inhibition effect of TIMP on metalloproteinases (MMPs). Since the inhibited MMPs led to an inferior MSCs adhesion and proliferation, we considered that the appropriate degradation by MMPs would generate more bioactive domains and improve the cell microenvironment. Immunofluorescence staining further proved that the distribution of vitronectin was highly related to MMP-1 and MMP-2. It could be concluded that the difference of advanced structure in scaffold materials would be amplified to significant difference of multiple biological cell-matrix interactions, and finally led to different cellular fates.

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Publication details

The article was received on 05 Sep 2017, accepted on 02 Dec 2017 and first published on 04 Dec 2017

Article type: Paper
DOI: 10.1039/C7TB02377D
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    Collagen structure regulates MSCs behavior by MMPs involved cell-matrix interactions

    Y. Ni, Z. Tang, J. Yang, Y. Gao, H. Lin, L. Guo, K. Zhang and X. Zhang, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB02377D

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