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A new colorimetric and fluorescent probe with a large stokes shift for rapid and specific detection of biothiols and its application in living cells

Abstract

In this work, a new reversible colorimetric and fluorescent probe for sequential recognition of copper ions and biothiols is synthesized easily. Based on the chelation–enhanced fluorescence quenching (CHEQ) effect, this probe shows high sensitivity and selectivity towards Cu2+, which can be detected by naked eyes. And this experimental phenomenon can be recovered upon addition of biothiols, restoring its initial fluorescence intensity. This probe also features a very high response speed (less than 5 seconds) and a large stokes shift (178 nm) toward Cu2+ and biothiols. Moreover, the detection limit for Cu2+ and biothiols are as low as 7.34 nM and 10.3 nM, respectively. Additionally, this ON−OFF−ON–type fluorescence recognition circle can be repeated more than 5 times by addition of Cu2+ and biothiols in turn. Particularly, this 1−Cu2+ ensemble is further successfully applied for GSH detection in living cells.

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Publication details

The article was received on 30 Aug 2017, accepted on 27 Sep 2017 and first published on 27 Sep 2017


Article type: Paper
DOI: 10.1039/C7TB02323E
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    A new colorimetric and fluorescent probe with a large stokes shift for rapid and specific detection of biothiols and its application in living cells

    M. Zhang, H. Han, S. Zhang, C. Wang, Y. Lu and W. Zhu, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB02323E

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