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A microfluidic chip with double-sided herringbone microstructures for enhanced capture of rare tumor cells

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Abstract

A microfluidic chip with single-sided herringbone microstructure has been developed to isolate circulating tumor cells (CTCs) from blood samples of cancer patients. Here, we describe a new double-sided herringbone chip in which staggered herringbone micromixers are placed on both top and bottom surfaces of microchannels. The double-sided herringbone structure enables a high CTC capture efficiency of whole blood samples without depletion of red blood cells because of the effects of leukocyte margination and plasma skimming. However, compared with the traditional single-sided herringbone chip, the double-sided herringbone chip has more complicated geometrical design, leading to a difficulty in experimental optimization of geometrical parameters. In this study, we developed an analytical model to geometrically optimize the herringbone chip by investigating the interactions between cells and antibody-immobilized device surfaces for enhancing CTC capture efficiency. On-chip cell capture experiments for validating modeling results were performed by spiking cultured EpCAM-positive tumor cells into blood samples from healthy donors. Based on the geometrical parameters optimized from the single-sided herringbone chip, the geometrically optimized double-sided herringbone chip enables a capture efficiency of 94 ± 4% of rare tumor cells directly from whole blood.

Graphical abstract: A microfluidic chip with double-sided herringbone microstructures for enhanced capture of rare tumor cells

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Publication details

The article was received on 30 Aug 2017, accepted on 03 Nov 2017 and first published on 03 Nov 2017


Article type: Paper
DOI: 10.1039/C7TB02318A
Citation: J. Mater. Chem. B, 2017, Advance Article
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    A microfluidic chip with double-sided herringbone microstructures for enhanced capture of rare tumor cells

    M. Wang, Z. Wang, M. Zhang, W. Guo, N. Li, Y. Deng and Q. Shi, J. Mater. Chem. B, 2017, Advance Article , DOI: 10.1039/C7TB02318A

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