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In-situ synthesis of fluorescent polydopamine nanoparticles coupled with enzyme-controlled dissolution of MnO2 nanoflakes for sensitive immunoassay of cancer biomarkers

Abstract

Herein, a novel immunosensing strategy was designed for sensitive fluorescence/visual detection of alpha-fetoprotein (AFP) by combining with enzyme-controlled formation of polydopamine (PDA) nanoparticles and dissolution of manganese dioxide (MnO2) nanoflakes. Initially, a sandwich-type immunoreaction was fabricated on the capture antibody-coated microplate by using detection antibody/ascorbate oxidase-conjugating gold nanoparticles (AOX-AuNP-Ab2) as the detection probe. The carried ascorbate oxidase, following by the immunocomplex, oxidized ascorbic acid (AA) into dehydroascorbic acid (DAA) to inhibit the dissolution of MnO2 nanoflakes, thereby resulting in the increment of fluorescent polydopamine nanoparticles by oxdizing dopamine into quinone derivative with the aid of MnO2 nanoflakes. Under toptimal conditions, the fluorescence intensity of PDA nanoparticles increased with the increasing AFP concentration within the dynamic working range of 0.05 – 20 ng/mL with a detection limit (LOD) of 17.3 pg/mL. The sensor also showed high specificity and acceptable accuracy for the detection of target AFP against other potential cancer biomarkers. Importantly, this study demonstrated for the first time that fluorescent PDA nanoparticles could be used as the signal-generation tags and applied to enzyme immunoassay. Despite this methodology focusing on target AFP detection, it is suitable for detection of other cancer biomarkers by controlling the corresponding antibody.

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Publication details

The article was received on 28 Aug 2017, accepted on 06 Oct 2017 and first published on 07 Oct 2017


Article type: Paper
DOI: 10.1039/C7TB02291C
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    In-situ synthesis of fluorescent polydopamine nanoparticles coupled with enzyme-controlled dissolution of MnO2 nanoflakes for sensitive immunoassay of cancer biomarkers

    Z. Lin, M. Li, S. Lv, K. Zhang, M. Lu and D. Tang, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB02291C

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