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Tailored Biomimetic Hydrogel Based on a Photopolymerised DMP1/MCF/Gelatin Hybrid System for Calvarial Bone Regeneration

Abstract

Searching effective osteoinduction factors with higher specificity and biosafety for the preparation of biomimetic materials, which mimic the natural bone extracellular matrix (ECM), seems to be an optimum strategy for achieving the ideal bone regeneration. Dentin matrix protein 1 (DMP1) seems to be a highly promising candidate due to its pleiotropic bio-regulation roles in several bone formation processes including osteoinductive, osteogenesis and biomineralization. In this study, we first generated a novel meso/macro-structured photopolymerised hybrid hydrogel system, in which DMP1 was loaded in mesocellular silica foam (MCF) and then evenly embedded in the photopolymerised gelatin hydrogel. This DMP1 loaded hybrid system (DMP1/MCF/Gel) exhibited hierarchical porous structure according to the surface area and pore size distribution analysis, TEM and SEM. Protein loading quantification and release kinetics experiments demonstrated that DMP1 is well protected and stably released for a longer duration in the DMP1/MCF/Gel system. The expression levels of bone markers in the BMSCs under the co-treatment of both Si ion and DMP1 suggested there was a synergy effect between Si ion and DMP1 what released from DMP1/MCF/Gel system. Further in vitro osteoinductive experiments of bone marrow stem cells (BMSCs) showed that the DMP1/MCF/Gel system enhanced the activity of alkaline phosphatase (ALP), promoted the formation of mineralized bone nodules, and up-regulated the expression levels of several osteogenic markers (including Runx2, Osx, Bsp, Ocn, Opn, Dmp1). In addition, both μ-CT and histological studies suggested that the in vivo repairment of rat calvarial defects using the DMP1/MCF/Gel system led to an accelerated bone regeneration with better mineralization and higher quality. In summary, this work generated a tailored biomimetic hydrogel based on a photopolymerised DMP1/MCF/Gel hybrid system, which mimicked the non-mineralized bone ECM and resulted in an improved bone regeneration.

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Publication details

The article was received on 09 Aug 2017, accepted on 06 Nov 2017 and first published on 07 Nov 2017


Article type: Paper
DOI: 10.1039/C7TB02130E
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    Tailored Biomimetic Hydrogel Based on a Photopolymerised DMP1/MCF/Gelatin Hybrid System for Calvarial Bone Regeneration

    S. Lin, L. Cao, Q. Wang, J. du, D. Jiao, J. Wu, Q. Gan and X. Jiang, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB02130E

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