Jump to main content
Jump to site search

Issue 44, 2017
Previous Article Next Article

Rapid synthesis of nitrogen doped carbon dots and their application as a label free sensor array for simultaneous discrimination of multiple proteins

Author affiliations

Abstract

In this work, we develop a facile one-step strategy for rapidly (within 1 min) preparing four kinds of nitrogen doped carbon dots (N-CDs) and construct a label free sensor array for protein discrimination. The monodispersed blue emitting N-CDs, with a quantum yield (QY) of about 7.6% (calibrated with quinine sulfate as the reference), were systematically characterized by using fluorescence spectroscopy, atomic force microscopy (AFM), dynamic light scattering (DLS), X-ray photoelectron spectroscopy (XPS) and infrared spectroscopy (IR). Based on the diverse fluorescence intensity response patterns, eight proteins with diverse isoelectric points and molecular weights could be simultaneously and effectively discriminated at nanomolar concentration by linear discrimination analysis (LDA). Proteins with different concentrations or protein mixtures can also be well identified. Furthermore, multiple proteins could still be well identified without any overlap in the presence of human urine. Moreover, serums from rectal cancer patients, Alzheimer's disease patients and healthy people could be effectively discriminated, showing great potential in auxiliary clinical diagnosis.

Graphical abstract: Rapid synthesis of nitrogen doped carbon dots and their application as a label free sensor array for simultaneous discrimination of multiple proteins

Back to tab navigation

Supplementary files

Publication details

The article was received on 09 Aug 2017, accepted on 05 Oct 2017 and first published on 10 Oct 2017


Article type: Paper
DOI: 10.1039/C7TB02129A
Citation: J. Mater. Chem. B, 2017,5, 8748-8753
  •   Request permissions

    Rapid synthesis of nitrogen doped carbon dots and their application as a label free sensor array for simultaneous discrimination of multiple proteins

    S. Xu, Z. Su, Z. Zhang, Y. Nie, J. Wang, G. Ge and X. Luo, J. Mater. Chem. B, 2017, 5, 8748
    DOI: 10.1039/C7TB02129A

Search articles by author

Spotlight

Advertisements