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Rapid synthesis of nitrogen doped carbon dots and their application as label free sensor array for simultaneous discrimination of multiple proteins

Abstract

In this work, we develop a facile one-step strategy for rapidly (within 1 min) preparing four kinds of nitrogen doped carbon dots (N-CDs) and construct a label free sensor array for protein discrimination. The monodispersed blue emitting N-CDs, with a quantum yield (QY) of about 7.6 % (calibrated with quinine sulfate as the reference), were systematic characterized by using fluorescence spectra, atomic force microscopy (AFM), dynamic light scattering (DLS), X-ray photoelectron spectroscopy (XPS) and infrared spectra (IR). Based on diverse fluorescence intensity responses patterns, eight proteins with diverse isoelectric points and molecular weights could be simultaneously and effectively discriminated at nanomolar concentration by Linear discrimination analysis (LDA). Proteins with different concentrations or protein mixtures can also be well identified. Furthermore, multiple proteins could still be well identified without any overlap in the presence of human urine sample. Moreover, serums from rectal cancer patients, alzheimei’s disease patients and healthy people could be effectively discriminated, showing great potential in auxiliary clinical diagnosis.

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Publication details

The article was received on 09 Aug 2017, accepted on 05 Oct 2017 and first published on 10 Oct 2017


Article type: Paper
DOI: 10.1039/C7TB02129A
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    Rapid synthesis of nitrogen doped carbon dots and their application as label free sensor array for simultaneous discrimination of multiple proteins

    S. Xu, Z. Su, Z. Zhang, Y. Nie, J. Wang, G. Ge and X. Luo, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB02129A

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