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Fluorescent microspheres for one-photon and two-photon imaging of mesenchymal stem cells

Abstract

Stem cell-mediated therapy has emerged as a novel regenerative treatment for tissue repair in the last decade. However, noninvasive monitoring the stem cells remains a grand challenge for stem cell-based regeneration medicine in full understanding the function and the behaviors of cells. Herein, acridine orange, a fluorescent dye was encapsulated into polymer nanospheres based on double emulsions. The fluorescent nanospheres with a diameter of around 200 nm were incubated with human mesenchymal stem cells (hMSCs) to produce nanosphere-endocytosed hMSCs. The fluorescent imaging of hMSCs can be directly and clearly captured using confocal microscopy in one-photon and two-photon modes. The results indicated that the hMSCs adhered and spread well on the surface of the scaffolds with a high population and good distribution. In the meanwhile, polystyrene microspheres were stained with dye complexes by using gradual solvent evaporation method. The as-prepared fluorescent beads exhibited bright and multiple colors and broad emission spectrum ranging from 500 to 800 nm, which was further used to quantitatively evaluate the one-photon and two-photon fluorescent signals of hMSCs. Our study offers a possibility of direct monitoring stem cells with high resolution, and encourages the future quantitative clinical assessment in imaging-guided cell therapies.

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Publication details

The article was received on 19 Jul 2017, accepted on 12 Sep 2017 and first published on 12 Sep 2017


Article type: Paper
DOI: 10.1039/C7TB01942D
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    Fluorescent microspheres for one-photon and two-photon imaging of mesenchymal stem cells

    Q. Zhang, J. Nie, H. Xu, Y. Qiu, X. Li, W. Gu, G. Tang and J. Luo, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB01942D

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