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Silicate-based bioceramics regulating osteoblast differentiation through BMP2 signalling pathway

Abstract

Bioactive materials with osteostimulation properties have the potential to promote bone regeneration. We have found that silicate-based biomaterials have osteostimulation ability for regeneration of large bone defects; however, the corresponding mechanism is unclear. In this study, we set out to elucidate the potential mechanism of silicate-based biomaterials with osteostimulation ability. A model silicate bioceramic, nagelschmidtite (NAGEL, Ca7P2Si2O16), was applied to study their ionic products on the effect of Bone morphogenic protein (BMP) signaling pathway for osteoblast MC3T3-E1 as NAGEL has been previously shown excellent in vitro and in vivo bone-forming activity. BMP signaling, especially BMP2, is involved in bone formation during mammalian development and exhibits versatile regulatory functions in the body. It is found that NAGEL bioceramics significantly enhance the migration and osteoblastic differentiation of MC3T3-E1. mRNA and protein expression of BMP2 is enhanced by NAGEL bioceramics in a dose-dependent manner. Moreover, NAGEL bioceramics activate Smad-dependent BMPs signaling pathway and induce the activation of BMP downstream cascade (OCN, OPN and Runx2). The accumulation of phosphorylated-Smad1/5 is induced by NAGEL bioceramics in MC3T3-E1 cell nucleus. It is further found that NAGEL bioceramics-mediated migration, osteoblastic differentiation and the activation of BMP downstream cascade are significantly downregulated by inhibition of BMP2 activity. Our results suggest that silicate-based NAGEL bioceramics possess excellent in vitro osteostimulation property and the possible mechanism of silicate-based biomaterials with distinct osteostimulation may be directly related to the activation of BMP2 signaling pathway of osteoblasts by release of Si-containing bioactive ionic products.

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Publication details

The article was received on 18 Jul 2017, accepted on 09 Aug 2017 and first published on 09 Aug 2017


Article type: Paper
DOI: 10.1039/C7TB01931A
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    Silicate-based bioceramics regulating osteoblast differentiation through BMP2 signalling pathway

    D. Zhai, M. Xu, L. Liu, J. Chang and C. Wu, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB01931A

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