Issue 40, 2017

Antibody-modified hydroxyapatite surfaces for the efficient capture of bladder cancer cells in a patient's urine without recourse to any sample pre-treatment

Abstract

In this study, we describe a sensitive protocol for the detection of bladder cancer cells in a patient's urine without pre-treatment of the urine sample using antibody-modified hydroxyapatite (HAp) micro/nanostructured surfaces converted from natural seashells under mild biomineralization conditions. The mechanism based on fractal dimension-crystalline phase interactions was utilised for the enhancement of cell capture capabilities that has been verified by application in the determination of 22 urine samples from bladder cancer patients. We have established a facile and economical yet biocompatible and bioactive surface with a topography consisting of micro and nano features that provide useful binding sites for antibodies permitting the rapid identification of cancer cells during urine cytology via fluorescence observation with the naked eye.

Graphical abstract: Antibody-modified hydroxyapatite surfaces for the efficient capture of bladder cancer cells in a patient's urine without recourse to any sample pre-treatment

Supplementary files

Article information

Article type
Paper
Submitted
09 Jul 2017
Accepted
15 Aug 2017
First published
22 Aug 2017
This article is Open Access
Creative Commons BY license

J. Mater. Chem. B, 2017,5, 8125-8132

Antibody-modified hydroxyapatite surfaces for the efficient capture of bladder cancer cells in a patient's urine without recourse to any sample pre-treatment

W. Zhang, K. Zhao, C. E. Banks and Y. Zhang, J. Mater. Chem. B, 2017, 5, 8125 DOI: 10.1039/C7TB01854A

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