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Antibody-Modified Hydroxyapatite Surfaces for the Efficient Capture of Bladder Cancer Cells in Patient’s Urine without Recourse to Any Sample Pre-treatment

Abstract

In this study, we describe a sensitive protocol for the detection of bladder cancer cells in patient’s urine without pre-treatment of the urine sample using antibody-modified hydroxyapatite (HAp) micro/nanostructured surfaces converted from natural seashell under mild biomineralisation conditions. The mechanism based on fractural dimension-crystalline phase interactions was utilised for the enhancement of cell capture capabilities that has been verified by the application in the determination of 22 urine samples from bladder cancer patients. We have established a facile and economical yet biocompatible and bioactive surface with a topography consisting of micro and nano features that provide useful binding sites for antibodies permitting the rapid identification of cancer cells during urine cytology via fluorescent observation with the naked eye.

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Publication details

The article was received on 09 Jul 2017, accepted on 15 Aug 2017 and first published on 22 Aug 2017


Article type: Paper
DOI: 10.1039/C7TB01854A
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
  • Open access: Creative Commons BY license
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    Antibody-Modified Hydroxyapatite Surfaces for the Efficient Capture of Bladder Cancer Cells in Patient’s Urine without Recourse to Any Sample Pre-treatment

    C. E. Banks, W. Zhang, K. Zhao and Y. Zhang, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB01854A

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