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Biomaterials for Polynucleotide Delivery to Anchorage-Independent Cells

Abstract

Anchorage-independent cells possess morphological features and cell membrane compositions distinct from adherent cells. They display minimal surface area, have a low rate of endocytosis and generally possess little proteoglycans that make it a challenge to deliver nucleic acids into them. A wide range of methods and materials have been developed to tackle the delivery obstacles for the polynucleotide-based therapeutics in modifying non-adherent cells. This article summarizes the techniques and biomaterials that have been utilized for transfection of anchorage-independent cells. Physical techniques are first briefly described along with particular applications for which they are well-suited. The structure-activity relationship of various biomaterial carriers of polynucleotides are then discussed with strategies employed to enhance their capability to transfect anchorage-independent cells. In conclusion, authors’ perspectives on different methods for polynucleotide delivery to primary human cells are compared, along with a discussion of their progression towards clinical trials.

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Publication details

The article was received on 07 Jul 2017, accepted on 09 Aug 2017 and first published on 09 Aug 2017


Article type: Review Article
DOI: 10.1039/C7TB01833A
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    Biomaterials for Polynucleotide Delivery to Anchorage-Independent Cells

    A. S. Ansari, J. P. Santerre and H. Uludag, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB01833A

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