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Fabrication of Photo-electrochemical biosensor for ultrasensitive screening of mono-bioactive molecules: effect of geometrical structures and crystal surfaces

Abstract

The controlled design of biosensors based on photo-electrochemical technique with high selectivity, sensitivity, and rapid response for monitoring of mono-bioactive molecules, particularly dopamine (DA) levels in neuronal cells is highly necessary for clinical diagnosis. Hierarchical carbon-, nitrogen-doped (CN) nickel oxide spear thistle (ST) flowers associated in single-head (S), and symmetric and asymmetric-double heads (D, and A, respectively) that tightly connected through micrometric dipole-like rod or trunk were fabricated by using a simple synthetic protocol. The CN-ST flower heads were decorated with dense nano-tubular like hedgehog needless skins in vertical alignments. These designated architectures are key features for creating biosensor surface electrodes for photo-electrochemical, ultrasensitive screening of mono-bioactive molecules. The exceptional electrode designs produced numerous catalytically active sites, large surface area, and high electron-transfer mobility. The active coating of carbon-nitrogen nanospheres significantly enhanced the photo-electrocatalytic activity of the prepared biosensor electrodes and prevented leakage of photocatalytic activity under long-term exposure to irradiation. Among all photo-electrochemical assays, the biosensors showed significant sensitivity and selectivity of DA in the presence of interfered molecules such as ascorbic acid (AA), uric acid (UA), adrenaline (A), and noradrenaline (NA). The photo-electrochemical of the CN-SST-{110} crystal surface electrode showed significant sensing performance of DA in terms of unimpeded diffusion pathways, a wide concentration-detection range, and a low detection limit, even in the coexistence of the potentially interfering molecules compared with other electrodes-modified CN-DST-{111} and CN-AST-{101} crystal surfaces. Furthermore, the CN-SST photo-biosensor electrode shows potential in the selective and sensitive determination of DA in real samples, such as human serum and secreted DA from living cells. This finding indicates that the hierarchical ST biosensor may enable analytical discrimination and monitoring of DA and can be employed for clinical diagnosis application.

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Publication details

The article was received on 04 Jul 2017, accepted on 08 Sep 2017 and first published on 08 Sep 2017


Article type: Paper
DOI: 10.1039/C7TB01803G
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    Fabrication of Photo-electrochemical biosensor for ultrasensitive screening of mono-bioactive molecules: effect of geometrical structures and crystal surfaces

    N. Akhtar, M. Y. Emran, M. Shenashen, H. Khalifa, T. Osaka, A. Faheem, T. Homma, H. Kawarada and S. El-Safty, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB01803G

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