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Transfection-capable polycationic nanovectors which include PEGylated-cyclodextrin structural units. A new synthesis pathway.

Abstract

Efficient tools are still searched to substitute the viral vectors in nucleic acids delivery applications. One of the most severe constraint in producing them is related to the strict reproducibility of their molecular characteristics, ensured by synthesis. In this work, we report an original route to obtain polycationic nanoentities with low variability, able to act as cooperating carriers, for dsDNA complexation and transport. The carriers are synthesized by rigorous conjugation of β-cyclodextrin (β-CD) with precise ratios of 2 kDa branched poly(ethyleneimine) (b-PEI), and 0.75 kDa poly(ethylene glycol) (PEG). The low cytotoxicity was the main designed parameter, beside the highest possible transfection ability, both of them proved on HeLa cells culture. A reporter gene which induces the expression of green-fluorescent-protein (GFP), inserted in a plasmid, was used to perform the necessary quantitations. In silico molecular modelling guided the carrier design, and confirmed the functional mimicry of histones, in tight and compact nucleosome-like spiral packaging of dsDNA. The synthesized reproducible carrier molecules are expected to largely confirm their feasible application in gene transfection.

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Publication details

The article was received on 23 Jun 2017, accepted on 07 Aug 2017 and first published on 07 Aug 2017


Article type: Paper
DOI: 10.1039/C7TB01722G
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    Transfection-capable polycationic nanovectors which include PEGylated-cyclodextrin structural units. A new synthesis pathway.

    A. Dascalu, A. Rodinel, A. Neamtu, S. Maier, C. M. Uritu, A. Nicolescu, S. Mihaela, D. Peptanariu, M. Calin and M. Pinteala, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB01722G

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