Jump to main content
Jump to site search

Issue 34, 2017
Previous Article Next Article

Devising new lipid-coated calcium phosphate/carbonate hybrid nanoparticles for controlled release in endosomes for efficient gene delivery

Author affiliations

Abstract

Lipid-coated calcium phosphate (LCP) nanoparticles (NPs) are proven to be effective vehicles for the delivery of genes and some drugs, while it is not desirable for NPs to release genes/drugs in late endosomes/lysosomes. To achieve the early endosomal release and escape, we have designed and developed new lipid-coated calcium carbonate/phosphate (LCCP) hybrid NPs. These new hybrid LCCP NPs have a spherical structure with an average diameter of 40 nm and high gene loading capacity. We particularly demonstrate that the loaded dsDNA/siRNA is mostly released under mildly acidic conditions (pH 6.0–5.5). LCCP NPs are also effectively internalized by B16F10 cells in a dose and time dependent way. The delivery efficacy has been further demonstrated using two functional siRNAs, i.e. programmed death ligand 1 (PD-L1) siRNA for PD-L1 silencing and polo-like kinase 1 (PLK1) siRNA for growth inhibition of B16F10. Consistently, the LCCP loaded PD-L1 siRNA shows quicker PD-L1-mRNA inhibition than LCP NPs, indicating that LCCP NPs improved the siRNA release in endosomes.

Graphical abstract: Devising new lipid-coated calcium phosphate/carbonate hybrid nanoparticles for controlled release in endosomes for efficient gene delivery

Back to tab navigation

Supplementary files

Publication details

The article was received on 15 Jun 2017, accepted on 07 Aug 2017 and first published on 09 Aug 2017


Article type: Paper
DOI: 10.1039/C7TB01635B
Citation: J. Mater. Chem. B, 2017,5, 7194-7203
  •   Request permissions

    Devising new lipid-coated calcium phosphate/carbonate hybrid nanoparticles for controlled release in endosomes for efficient gene delivery

    Y. Wu, W. Gu, J. Tang and Z. P. Xu, J. Mater. Chem. B, 2017, 5, 7194
    DOI: 10.1039/C7TB01635B

Search articles by author

Spotlight

Advertisements