Jump to main content
Jump to site search


Devising New Lipid-coated Calcium Phosphate/Carbonate Hybrid Nanoparticles to Control Release in Endosome for Efficient Gene Delivery

Abstract

Lipid-coated calcium phosphate (LCP) nanoparticles (NPs) are proven to be an effective vehicle for gene and some drug delivery, while it is not desirable for NPs to release gene/drug in late endosome/lysosome. To achieve the early endosome release and escape, we have designed and developed new lipid-coated calcium carbonate/phosphate (LCCP) hybrid NPs. The new hybrid LCCP NPs have a spherical structure with an average diameter of 40 nm and a high gene loading capacity. We particularly demonstrate that the loaded dsDNA/siRNA is mostly released under mildly acidic conditions (pH 6.0-5.5). LCCP NPs are also effectively internalized by B16F10 cells in a dose and time dependent way. The delivery efficacy has been further demonstrated using two functional siRNAs, i.e. programmed death ligand 1 (PD-L1) siRNA for PD-L1 silencing and polo-like kinase 1 (PLK1) siRNA for growth inhibition of B16F10. In consistence, the LCCP loaded PD-L1 siRNA shows a quicker PD-L1-mRNA inhibition than LCP NPs, indicating that LCCP NPs improved the siRNA release in endosome.

Back to tab navigation

Supplementary files

Publication details

The article was received on 15 Jun 2017, accepted on 07 Aug 2017 and first published on 09 Aug 2017


Article type: Paper
DOI: 10.1039/C7TB01635B
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
  •   Request permissions

    Devising New Lipid-coated Calcium Phosphate/Carbonate Hybrid Nanoparticles to Control Release in Endosome for Efficient Gene Delivery

    Y. Wu, W. Gu, J. Tang and Z. P. Xu, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB01635B

Search articles by author

Spotlight

Advertisements