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Ratiometric fluorescence imaging of endogenous selenocysteine in cancer cell matrix

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Abstract

Monitoring intracellular selenocysteine (Sec) is of significant interest for studying Sec metabolism and disease-relevant changes in Sec homeostasis. Herein, we rationally designed an ICT-based ratiometric probe (Rat-Sec) for selectively discriminating endogenous Sec in a live cell matrix. By utilizing an acrylate moiety, Rat-Sec manifested significantly ratiometric and red-shifted (∼117 nm) fluorescence signals within 1 min, and concomitantly demonstrated apparent color alteration from colorless to faint yellow in the presence of Sec. When applied to quantitatively detect Sec under physiological conditions, Rat-Sec gives a detection limit of 12 nM. Offering great advantages due to its ratiometric nature and high specificity and selectivity, Rat-Sec provides reliable fluorescence quantification and rapid tracking of exogenous and endogenous Sec in living cells.

Graphical abstract: Ratiometric fluorescence imaging of endogenous selenocysteine in cancer cell matrix

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Publication details

The article was received on 08 Jun 2017, accepted on 14 Jul 2017 and first published on 14 Jul 2017


Article type: Paper
DOI: 10.1039/C7TB01558E
Citation: J. Mater. Chem. B, 2017, Advance Article
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    Ratiometric fluorescence imaging of endogenous selenocysteine in cancer cell matrix

    Y. Tian, F. Xin, C. Gao, J. Jing and X. Zhang, J. Mater. Chem. B, 2017, Advance Article , DOI: 10.1039/C7TB01558E

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