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Ratiometric fluorescent imaging for endogenous selenocysteine in cancer cell matrix

Abstract

Monitoring intracellular selenocysteine (Sec) is of significant interest for studying Sec metabolism and disease-relevant changes in Sec homeostasis. Herein we rationally designed an ICT-based ratiometric probe (Rat-Sec) for selectively discriminating endogenous Sec in live cell matrix. By utilizing an acrylate moiety, Rat-Sec manifested significantly ratiometric and red-shifted (~117 nm) fluorescent signals within 1 min, and concomitantly demonstrated apparent color alteration from colorless to faint yellow in the presence of Sec. When applied to quantitatively detect Sec under physiological conditions, Rat-Sec gives a detection limit of 12 nM. Offering great advantages due to its ratiometric nature and high specificity and selectivity, Rat-Sec provides us reliable fluorescent quantification and rapid tracking of exogenous and endogenous Sec in living cells.

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Publication details

The article was received on 08 Jun 2017, accepted on 14 Jul 2017 and first published on 14 Jul 2017


Article type: Paper
DOI: 10.1039/C7TB01558E
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    Ratiometric fluorescent imaging for endogenous selenocysteine in cancer cell matrix

    Y. Tian, F. Xin, C. Gao, J. Jing and X. Zhang, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB01558E

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