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Highly sensitive electrochemiluminescent detection of prostate cancer biomarker

Abstract

Prostate-specific membrane antigen (PSMA), a glycoprotein expressed in the prostatic epithelium endowed with enzymatic activity, is a very promising diagnostic marker for the early detection of prostate cancer. In this study, we report a novel electrochemiluminescence ELISA-like immunosensor based on carbon nanotubes and a highly specific sandwich immunoassay for the PSMA detection. To fabricate the device, an optically transparent electrode was modified with doubly functionalized multi-walled carbon nanotubes carrying amine groups and a monoclonal anti-PSMA antibody. Subsequently, to complete the sandwich immunosensing device, a second specific monoclonal anti-PSMA antibody was labelled with a electrochemiluminescent probe. Under optimized experimental conditions, the proposed sensing device exhibits a performance exceed that of state of-the-art in terms of limit of detection (LOD) and of quantification (LOQ) as good as 0.88 ng mL-1 and 2.60 ng mL-1, respectively, in real complex samples such as cell lysates. In addition, the unique role of carbon nanotubes is also discussed by comparison with an analogue sensor assembled without the nanocarbon-based material

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Supplementary files

Publication details

The article was received on 08 Jun 2017, accepted on 11 Jul 2017 and first published on 11 Jul 2017


Article type: Paper
DOI: 10.1039/C7TB01557G
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    Highly sensitive electrochemiluminescent detection of prostate cancer biomarker

    A. Juzgado, A. Soldà, A. Ostric, A. Criado, G. VALENTI, S. Rapino, G. Conti, G. Fracasso, F. Paolucci and M. Prato, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB01557G

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