Jump to main content
Jump to site search


Production of a fluorescence resonance energy transfer (FRET) biosensor membrane for microRNA detection

Author affiliations

Abstract

MicroRNAs (miRNAs) play a key role in regulating gene expression but can be associated with abnormalities linked to carcinogenesis and tumor progression. Hence there is increasing interest in developing methods to detect these non-coding RNA molecules in the human circulation system. Here, a novel FRET miRNA-195 targeting biosensor, based on silica nanofibers incorporated with rare earth-doped calcium fluoride particles (CaF2:Yb,Ho@SiO2) and gold nanoparticles (AuNPs), is reported. The formation of a sandwich structure, as a result of co-hybridization of the target miRNA which is captured by oligonucleotides conjugated at the surface of CaF2:Yb,Ho@SiO2 fibers and AuNPs, brings the nanofibers and AuNPs in close proximity and triggers the FRET effect. The intensity ratio of green to red emission, I541/I650, was found to decrease linearly upon increasing the concentration of the target miRNA and this can be utilized as a standard curve for quantitative determination of miRNA concentration. This assay offers a simple and convenient method for miRNA quantification, with the potential for rapid and early clinical diagnosis of diseases such as breast cancer.

Graphical abstract: Production of a fluorescence resonance energy transfer (FRET) biosensor membrane for microRNA detection

Back to tab navigation

Supplementary files

Publication details

The article was received on 23 May 2017, accepted on 31 Jul 2017 and first published on 01 Aug 2017


Article type: Paper
DOI: 10.1039/C7TB01399J
Citation: J. Mater. Chem. B, 2017, Advance Article
  •   Request permissions

    Production of a fluorescence resonance energy transfer (FRET) biosensor membrane for microRNA detection

    Y. Fu, T. Chen, G. Wang, T. Gu, C. Xie, J. Huang, X. Li, S. Best and G. Han, J. Mater. Chem. B, 2017, Advance Article , DOI: 10.1039/C7TB01399J

Search articles by author

Spotlight

Advertisements