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Chondrogenic Differentiation of BMSCs Encapsulated in Chondroinductive Polysaccharide/Collagen Hybrid Hydrogels

Abstract

Although BMSCs-based therapy is one of the most front-line technologies for cartilage repair, it is still a big challenge to attain ideal niches for BMSCs chondrogenic differentiation. In this study, we developed hyaluronate and chondroitin sulfate derivatives to prepare covalently crosslinked polysaccharide hydrogels. Based on these binary hydrogels, collagen was added to prepare ternary hybrid hydrogels and the effect on encapsulated BMSCs was studied. After culturing with different cell densities in vitro without the addition of growth factor for 3 weeks, the chondrogenesis of BMSCs was evaluated by CLSM, mechanical test, histological staining, immunohistochemical staining and gene expression. The results indicated that BMSCs in high cell density (50 million/mL) cell-laden constructs had more obvious chondrogenic phenotype than those in low cell density ones (5 million/mL). However, the components of hydrogels had significant influence on the chondrogenic differentiation. With the adding of collagen, the BMSCs in ternary hybrid hydrogels showed more significant chondrogenesis, possessing with more secreted glycoaminoglycans (GAGs) and collagen type II deposition, higher mechanical property and chondrogenic gene expression during 3 weeks culture in vitro. It can be concluded that the bioactive collagen is beneficial to the chondrogenesis of BMSCs. This hybrid hydrogels deserve further study to have a prospective application in tissue engineering for cartilage defect repair.

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Publication details

The article was received on 13 Apr 2017, accepted on 17 May 2017 and first published on 18 May 2017


Article type: Paper
DOI: 10.1039/C7TB01020F
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    Chondrogenic Differentiation of BMSCs Encapsulated in Chondroinductive Polysaccharide/Collagen Hybrid Hydrogels

    X. Li, Y. Teng, J. Liu, H. Lin, Y. Fan and X. Zhang, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB01020F

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