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Two-photon fluorescent probe for detecting cell membranal liquid-ordered phase by an aggregate fluorescence method

Abstract

Cell membranal liquid-ordered (Lo) phase controlled structure and function of the cell membranes. In this work, we have engineered a novel two-photon (TP) fluorescent probe, TP-HVC18, which remarkably displayed two different fluorescence emission profiles to the aggregate and solution states in the distinct polar environments. In accordance with its aggregate fluorescence, TP-HVC18 also can emit a red fluorescence signal in Lo phase vesicles. Taking advantage of this unique feature, we have demonstrated that the new TP probe TP-HVC18 is suitable for imaging membranal Lo phase by an aggregate fluorescence method. Furthermore, the robust probe also exhibited uncontinuous red fluorescence distribution in the cell membranal Lo phase. Based on this intriguing character, we also successfully showed that the novel probe can be employed to exhibit uncontinuous distribution of cell membranal Lo phase by 3D imaging technique. We expect that this aggregation-based fluorescent platform may be extended for the development of a wide variety of TP fluorescent probes for detecting many biological species.

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Publication details

The article was received on 10 Apr 2017, accepted on 09 May 2017 and first published on 09 May 2017


Article type: Paper
DOI: 10.1039/C7TB00979H
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    Two-photon fluorescent probe for detecting cell membranal liquid-ordered phase by an aggregate fluorescence method

    Y. Liu, F. Meng, J. Nie, J. Niu, X. Yu and W. Lin, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB00979H

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