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Issue 32, 2017
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Functionalization of bone implants with nanodiamond particles and angiopoietin-1 to improve vascularization and bone regeneration

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Abstract

One of the major challenges in bone tissue engineering is adequate vascularization within bone substituents for nutrients and oxygen supply. In this study, the production and results of a new, highly functional bone construct consisting of a commercial three-dimensional β-tricalcium phosphate scaffold (β-TCP, ChronOS®) and hydrophilic, functionalized nanodiamond (ND) particles are reported. A 30-fold increase in the active surface area of the ChronOS + ND scaffold was achieved after modification with ND. In addition, immobilization of angiopoietin-1 (Ang-1) via physisorption within the β-TCP + ND scaffold retained the bioactivity of the growth factor. Homogeneous distribution of the ND and Ang-1 within the core of the three-dimensional scaffold was confirmed using ND covalently labelled with Oregon Green. The biological responses of the β-TCP + ND scaffold with and without Ang-1 were studied in a sheep calvaria critical size defect model showing that the β-TCP + ND scaffold improved the blood vessel ingrowth and the β-TCP + ND + ND + Ang-1 scaffold further promoted vascularization and new bone formation. The results demonstrate that the modification of scaffolds with tailored diamond nanoparticles is a valuable method for improving the characteristics of bone implants and enables new approaches in bone tissue engineering.

Graphical abstract: Functionalization of bone implants with nanodiamond particles and angiopoietin-1 to improve vascularization and bone regeneration

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Publication details

The article was received on 15 Mar 2017, accepted on 31 May 2017 and first published on 31 May 2017


Article type: Paper
DOI: 10.1039/C7TB00723J
Citation: J. Mater. Chem. B, 2017,5, 6629-6636
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    Functionalization of bone implants with nanodiamond particles and angiopoietin-1 to improve vascularization and bone regeneration

    X. Wu, M. Bruschi, T. Waag, S. Schweeberg, Y. Tian, T. Meinhardt, R. Stigler, K. Larsson, M. Funk, D. Steinmüller-Nethl, M. Rasse and A. Krueger, J. Mater. Chem. B, 2017, 5, 6629
    DOI: 10.1039/C7TB00723J

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