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Issue 26, 2017
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A core–shell structured polyplex for efficient and non-toxic gene delivery

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Abstract

Cationic polymers are important vectors for gene transfection, but the paradox between high transfection efficacy and low toxicity is always a hindrance to their applications in gene therapy. Here, we developed a core–shell polyplex using a high generation dendrimer to condense plasmid DNA at a very low nitrogen/phosphorus (N/P) ratio, followed by coating with a low-molecular-weight cationic polymer to offset the positive charge. Different high generations of polyamidoamine (PAMAM) dendrimers as core polymers and low-molecular-weight linear polyethylenimine (LPEI), branched PEI and G2 PAMAM dendrimer as shell polymers were tested. Among them, the polyplex composed of G8 PAMAM dendrimer and LPEI (G8/LPEI/DNA) at a core N/P ratio of 0.25 and a shell N/P ratio of 100 exhibited the highest transfection efficacy and minimal toxicity. The mechanism investigation indicated that the G8/LPEI/DNA polyplex possessed an incompact structure that facilitated DNA release in the cytoplasm. Our study provides an ingenious approach for high efficiency and low toxicity gene transfection.

Graphical abstract: A core–shell structured polyplex for efficient and non-toxic gene delivery

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Publication details

The article was received on 13 Mar 2017, accepted on 02 May 2017 and first published on 03 May 2017


Article type: Paper
DOI: 10.1039/C7TB00690J
Citation: J. Mater. Chem. B, 2017,5, 5101-5108
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    A core–shell structured polyplex for efficient and non-toxic gene delivery

    S. Wang, F. Wang, Q. Zhang and Y. Cheng, J. Mater. Chem. B, 2017, 5, 5101
    DOI: 10.1039/C7TB00690J

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