Jump to main content
Jump to site search


Bioresponsive controlled glucose release from TiO2 nanotube arrays: A simple and portable biosensing system for cocaine with glucometer readout

Abstract

Herein we design a simple and portable biosensing platform for quantitative detection of cocaine based on target-triggered glucose release from TiO2 nanotube array (TiNTA) with glucometer readout. Initially, single-stranded DNA1 and DNA2 were covalently conjugated to the TiNTA and gold nanoparticle (AuNP), respectively. In the presence of cocaine aptamer and glucose, the immobilized DNA1 on the TiNTA and the labeled DNA2 on the AuNP simultaneously hybridized with the adjacent area of the aptamer, and gated the glucose molecules in the nanotubes. Upon cocaine introduction, the aptamer specifically reacted with analyte to form the target-aptamer complex, thus resulting in dissociation of DNA1-TiNTA and DNA2-AuNP to release glucose molecules from the nanotubes. The as-released glucose molecules could be determined on a personal glucometer (PGM). Experimental results revealed that the PGM signal increased with the increasing cocaine, and exhibited a wide linear range of 10 – 600 nM with a detection limit of 5.2 nM. Intra- and inter-assay relative standard deviations with identical batches were less than 9.3% and 12.7%, respectively. The specificity, reproducibility and stability of our strategy were acceptable. Importantly, this concept offers promise for rapid, simple, low-cost and user-friendly analysis of small-molecular cocaine.

Back to tab navigation

Publication details

The article was received on 10 Mar 2017, accepted on 13 Jun 2017 and first published on 15 Jun 2017


Article type: Paper
DOI: 10.1039/C7TB00670E
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
  •   Request permissions

    Bioresponsive controlled glucose release from TiO2 nanotube arrays: A simple and portable biosensing system for cocaine with glucometer readout

    D. Tang and G. Li, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB00670E

Search articles by author

Spotlight

Advertisements