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Extracellular Matrix Powder from Cultured Cartilage-Like Tissue as Cell Carriers for Cartilage Repair

Abstract

The cartilage ECM is a promising material for cartilage repair because of its bioactivity. However, the animal source of ECM unavoidably increases the risk of pathogen infection and the variability of product quality. In this study, we utilized a novel 3D culture method to prepare a new type of artificial decellularized matrix powder (DEMP) for the development of injectable, bioactive, biodegradable cell carriers for cartilage tissue engineering. This culture method combined hanging drop culture with suspension culture method, and was very efficient to produce cartilage-like tissue (CLT). By this method, the initial 2.3×106 chondrocytes generated as much as 58.22 mg wet weight CLT at two weeks, which proved to contain abundant glycoaminoglycans (GAGs), type II collagen and BMP-2, TGF-β1 growth factors by staining techniques and biochemical analysis. Subsequently, the two-week-old CLT was decellularized to prepare the artificial DEMP. In the in vitro study, it was found that MSCs cultured on DEMP differentiated to chondrocytes very well and secreted rich GAGs and type II collagen at three weeks even without exogenous TGF-β1. The in vivo study demonstrated that the DEMP not only facilitated regenerating the hyaline cartilage, which was implied by the intense staining of GAGs and type II collagen in rabbit subchondral defects at 1 month, but also benefited the regeneration of subchondral bone (bone ingrowth at 1 month: 48.22%) as shown in micro-CT data. Collectively, these results suggest that the artificially prepared DEMP by this culture method holds a great potential as a novel ECM material for cartilage repair.

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Publication details

The article was received on 08 Mar 2017, accepted on 29 Mar 2017 and first published on 29 Mar 2017


Article type: Paper
DOI: 10.1039/C7TB00640C
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    Extracellular Matrix Powder from Cultured Cartilage-Like Tissue as Cell Carriers for Cartilage Repair

    Y. Teng, X. Li, Y. Chen, H. Cai, W. Cao, X. Chen, Y. Sun, J. Liang, Y. Fan and X. Zhang, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB00640C

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