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Issue 21, 2017
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The improved sensitive detection of C-reactive protein based on the chemiluminescence immunoassay by employing monodispersed PAA-Au/Fe3O4 nanoparticles and zwitterionic glycerophosphoryl choline

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Abstract

Nanocomposites are emerging complex nanoscale materials composed of different phases that have received considerable attention as a result of their widespread impact on a myriad of fields. Having said this, it is important to recognize that poor colloidal stabilities in physiological media and non-specific interactions with biology are key limitations prohibiting their successful translation into the development of practical technologies with real impact. Herein, PAA coated Au/Fe3O4 composite magnetic nanoparticles (NPs) with monodispersity and high biostability were engineered for the development of a highly sensitive C-reactive protein (CRP) chemiluminescence immunoassay (CLIA), based on the horseradish peroxidase (HRP)–luminol–H2O2 system. In addition, zwitterionic glycerophosphoryl choline (GPC) was employed as a co-blocking agent to reduce the nonspecific adsorption. This assay reports a limit of detection (LOD) of 2.5 pM with high reproducibility verified clinically and demonstrating improved progress towards translation into clinics.

Graphical abstract: The improved sensitive detection of C-reactive protein based on the chemiluminescence immunoassay by employing monodispersed PAA-Au/Fe3O4 nanoparticles and zwitterionic glycerophosphoryl choline

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Publication details

The article was received on 07 Mar 2017, accepted on 24 Apr 2017 and first published on 24 Apr 2017


Article type: Paper
DOI: 10.1039/C7TB00637C
Citation: J. Mater. Chem. B, 2017,5, 3919-3926
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    The improved sensitive detection of C-reactive protein based on the chemiluminescence immunoassay by employing monodispersed PAA-Au/Fe3O4 nanoparticles and zwitterionic glycerophosphoryl choline

    Y. Xing, Q. Gao, Y. Zhang, L. Ma, K. Y. Loh, M. Peng, C. Chen and Y. Cui, J. Mater. Chem. B, 2017, 5, 3919
    DOI: 10.1039/C7TB00637C

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