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Development of a selective cell capture and release assay: impact of clustered RGD ligands

Abstract

There is a growing interest in isolating tumor cells from biological samples. Considering that circulating tumor cells can be rare in blood, it appears challenging to capture these cells onto a surface with high selectivity and sensitivity. In the present paper we describe the design of functionalized surfaces aiming to selectively capture tumor cells by using the RGD peptide ligand either with a tetrameric or a monomeric presentation. β-cyclodextrin-coated self-assembled monolayers were used as platforms for the binding of RGD ligands endowed with a redox ferrocene cluster as the dissociation of the inclusion complex on the surface is accounted for the release of the captured cells upon electrochemical oxidation of ferrocene. For this purpose, we determined suitable RGD densities for both monovalent and tetravalent ligand presentations. The present results indicate that the clustered RGD architecture efficiently improves the selective cell capture at a very low RGD-surface density (∼ 10 RGD/μm2) in comparison with the monovalent presentation (∼ 1000 RGD/ μm2).

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Publication details

The article was received on 07 Mar 2017, accepted on 16 May 2017 and first published on 16 May 2017


Article type: Paper
DOI: 10.1039/C7TB00630F
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    Development of a selective cell capture and release assay: impact of clustered RGD ligands

    M. Degardin, D. Thakar, M. Claron, R. Richter, L. Guerente and D. Boturyn, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB00630F

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